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  • Title: An optimized enzyme-linked immunosorbent assay for quantitative diagnosis of bovine fascioliasis.
    Author: Wyckoff JH, Bradley RE.
    Journal: J Parasitol; 1986 Jun; 72(3):439-44. PubMed ID: 3746564.
    Abstract:
    An optimized immunoassay for detection of antibody to Fasciola hepatica antigen in cattle was developed through the adaptation of a kinetics-dependent, enzyme-linked immunosorbent assay (k-ELISA) to a microplate format. Enhanced sensitivity and a strict quantitative nature were achieved with the utilization of enzyme kinetics. With this k-ELISA, significant (P less than 0.01) elevations in anti-F. hepatica antibody could be detected as early as 2 wk post-infection in experimentally infected calves. Furthermore, fluke-burden related differences in anti-F. hepatica antibody levels between 3 different levels of fluke infection were evident.
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