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  • Title: Protocol to isolate human normal and neoplastic pancreatic cells for single-cell omic analyses.
    Author: Chen H, Peng J, Liu L, Huang D, Zhao Y, Wu W.
    Journal: STAR Protoc; 2023 Sep 15; 4(3):102464. PubMed ID: 37480562.
    Abstract:
    The high-throughput sequencing at single-cell resolution requires high-quality samples of inputted 100-10,000 cells with at least 80%-90% viability according to the applied platform. Here, we present a protocol for single-cell isolation from normal and neoplastic human pancreas. We describe steps for sample harvesting, procurement, tissue digestion, and cell purification. Subsequently, isolated cells can be further applied to single-cell profiling, for example, single-cell RNA sequencing and single-cell ATAC-seq using 10× Genomics platform. For complete details on the use and execution of this protocol, please refer to Peng et al. (2019)1 and Li et al. (2021).2.
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