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  • Title: The isolation and genetic characteristics of lambda transducing phages of the uvrA+ and uvrC+ genes of E. coli K12.
    Author: Auerbach J, Howard-Flanders P.
    Journal: Mol Gen Genet; 1979 Jan 11; 168(3):341-4. PubMed ID: 374995.
    Abstract:
    Lambda transducing phages carrying the excision repair genes uvrA+ and uvrC+ were selected from a pool of lambda phages carrying EcoR1 fragments of E. coli DNA. These phages and also lambdauvrB+ (obtained from Gottesman) were used to make lysogens of excision-defective strains carrying uvrA-, uvrB- or uvrC-. Lambda uvrA+ was found to transduce strains carrying uvrA- but not those carrying uvrB- or uvrC-, to normal ultraviolet resistance. Similarly, lambdauvrB+ and lambdauvrC+ were found to complement only the corresponding uvr- allele. The lambda transducing phages were co-transduced with gal+ by P1 phage into lysogenic gal- recipients, and presumably were integrated at the normal prophage site.
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