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  • Title: Optimization of the production of hCG beta-like material by CaSki human cervical carcinoma cells in roller bottle culture.
    Author: Hussa RO, Pattillo RA, Ruckert AC, Rinke ML, Cortesi JP, Fein HG.
    Journal: Oncology; 1986; 43(1):54-9. PubMed ID: 3753621.
    Abstract:
    This study was aimed at optimizing large-scale roller bottle culture conditions for CaSki human cervical carcinoma cells, to produce ectopic hCG beta-like material in quantities sufficient for subsequent characterization studies. Several cell culture techniques contributed to the achievement of this goal: (1) use of serum-free culture medium; (2) use of intermittent recovery periods in presence of serum; and (3) ultrafiltration of the serum-free medium pool for initial concentration of 100-fold.
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