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  • Title: Sarcoplasmic reticulum interacts with the Ca(2+) indicator precursor fura-2-am.
    Author: Highsmith S, Bloebaum P, Snowdowne KW.
    Journal: Biochem Biophys Res Commun; 1986 Aug 14; 138(3):1153-62. PubMed ID: 3755905.
    Abstract:
    Fura-2-am, the pentaester precursor of the fluorescent Ca(2+) indicator fura-2, is modified when it is exposed to isolated skeletal muscle sarcoplasmic reticulum vesicles. The modified fura-2-am has enhanced fluorescence, is not sensitive to Ca(2+), and is partially bound to the SR membrane. The isolated product is further converted into fura-2 by esterase. It is suggested that the SR-induced modification is a selective enzymatic hydrolysis of only some of the five ester moieties on fura-2-am. A structure is proposed to account for the results. The potential for this effect of SR on fura-2-am to cause complications in measurements of in vivo intracellular free [Ca(2+)] is noted.
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