These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Surface antigens of virulent strains of Aeromonas hydrophila.
    Author: Dooley JS, Lallier R, Trust TJ.
    Journal: Vet Immunol Immunopathol; 1986 Jun; 12(1-4):339-44. PubMed ID: 3765354.
    Abstract:
    Antiserum was raised in rabbits to whole cells of a representative strain from a group of A. hydrophila strains exhibiting enhanced virulence for fish. The major surface antigens of the strain were identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. The lipopolysaccharide (LPS) was examined using SDS-PAGE and silver staining. It was found to possess O polysaccharide chains of homogeneous length that were highly immunogenic. The LPS was conserved both morphologically and antigenically throughout the high virulence group. Heat-labile protein antigens were detected after absorption of the antiserum with boiled cells of the homologous strain. Only one major protein antigen, with a molecular weight of approximately 52,000, was present in outer membrane preparations or in whole cell lysates. A representative strain from the high virulence group, strain TF7, was shown by electron microscopy to be covered by a regular surface protein array (S-layer) which was found to be composed primarily of the 52 KD protein antigen. All the other members of the A. hydrophila high virulence group were shown to possess similar S-layers.
    [Abstract] [Full Text] [Related] [New Search]