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  • Title: Evaluation of enzyme linked immunosorbent assay in the immunodiagnosis of schistosomiasis.
    Author: Hassan F, Abdel-Wahab MF, Nosseur A, Sedeek S, Shehata A, Masood MA.
    Journal: J Trop Med Hyg; 1979 Jan; 82(1):3-7. PubMed ID: 376867.
    Abstract:
    Eighty-six bilharzial patients divided into 5 clinical groups were studied. Enzyme linked immunosorbent assay (ELISA), indirect fluorescent antibody (IFA) and indirect haemagglutination (IHA) test were performed for all the patients. ELISA gave the most sensitive results (82.6% and 80.2% positivity rate in Egypt and Lille respectively), followed by IFA (79.1% positivity rate) and IHA (77.9% and 75.6% positivity rate in Egypt and Lille respectively). The humoral antibodies detected by all methods (ELISA, IFA and IHA) showed increasing values with the progress of the disease which is parallel to the antigenicity of the disease. Both the positivity rate and the mean value of antibody titres recorded by the 3 diagnostic techniques (ELISA, IFA and IHA) were significantly higher in mansoni than haematobium infection. This may be explained by species specificity of antibody response. The superiority of ELISA over IFA and IHA techniques was discussed.
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