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  • Title: Purification of bovine neurosecretory granule membranes by density gradient centrifugation.
    Author: González CB, Caorsi CE, North WG, Berrios OT.
    Journal: Anal Biochem; 1986 Sep; 157(2):316-22. PubMed ID: 3777435.
    Abstract:
    A procedure for the subfractionation of neurosecretory granules into membrane and content components is described. The procedure involves the hypotonic lysis of the secretory granule fraction and further purification of the membranes by centrifugation through a discontinuous sucrose gradient. The neurosecretory granule membranes represented 5.2% of the total proteins of the neurosecretory granule fraction and were highly enriched in cytochrome b561. Electron microscopic analysis of the purified membranes showed vesicles devoid of electrodense content.
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