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  • Title: Metabolic cooperation in vitro: differential ability of ouabain to uncouple normal, preneoplastic, and neoplastic mouse mammary cells.
    Author: Miller FR, McInerney D, Miller BE.
    Journal: Biochem Biophys Res Commun; 1986 Nov 14; 140(3):955-61. PubMed ID: 3778494.
    Abstract:
    We utilized two assays for metabolic cooperation in vitro, one in which cells were grown in monolayer and one in which the cells formed three-dimensional structures in a collagen gel matrix. Both assays required one of the test cell pair to be resistant both to ouabain and to thioguanine (deficient in hypoxanthine-guanine phosphoribosyltransferase). Normal mammary gland cells, cells from preneoplastic mouse hyperplastic alveolar nodules, and mouse mammary tumor cells were metabolically linked in vitro to the drug-resistant tumor cells. Ouabain abrogated communication between tumor cells and normal mammary gland cells and between tumor cells and preneoplastic cells but had no effect on communication between tumor cells.
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