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  • Title: Differential effects of nerve-growth factor and glioma-conditioned medium on neurons cultured from various regions of fetal rat central nervous system.
    Author: Hatanaka H, Tsukui H.
    Journal: Brain Res; 1986 Nov; 395(1):47-56. PubMed ID: 3779432.
    Abstract:
    When nerve-growth factor (NGF) was added to 17-day fetal rat central nervous system (CNS) septal neurons in culture using a defined medium, choline acetyltransferase (ChAT) activity was greatly induced. However, glioma-conditioned medium (GCM), which is expected to contain cholinergic neurotrophic factor(s) different from NGF, did not affect the ChAT activity of the cultured septal neurons. On the contrary, ChAT activity of 19-day fetal rat hippocampal neurons in culture was increased by the addition of GCM but not by NGF. These phenomena were confirmed in septal and hippocampal neuronal cultures obtained from the same embryonic day, i.e., 18-day fetal rat. The NGF-mediated increase in ChAT activity of cultured septal neurons was culture-time dependent (2.3-fold increase after 3 and 3.5-fold increase after 6 days in culture) and NGF-dose dependent (the ED50 value was 0.8 ng/ml). The effect of NGF was completely abolished by the addition of specific anti-NGF antibodies. The differential effects of NGF and GCM on several other cultured cholinergic neurons from 17-day fetal rat spinal cord, striatum, brainstem and amygdala were measured. NGF tended to increase ChAT activities of cultured striatal and amygdala neurons but not cultured spinal cord and brainstem neurons. GCM increased ChAT activities in the latter two cultures, while having no effect on the former cultures. Although the extent of increase of NGF-mediated ChAT activities of cultured striatal and amygdala neurons were low, NGF-mediated increase of the striatal ChAT activity showed the pattern resembling that of cultured septal neurons as to time-course, dose-dependency and anti-NGF antibody sensitivity. NGF did not affect the tyrosine hydroxylase activity in the cultured brainstem catecholaminergic neurons.
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