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  • Title: Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes.
    Author: Fuscoe JC, Clark LM, Van Dilla MA.
    Journal: Cytogenet Cell Genet; 1986; 43(1-2):79-86. PubMed ID: 3780319.
    Abstract:
    We report the construction of 15 human chromosome-specific DNA libraries. Metaphase chromosomes were purified by flow sorting and the DNA was extracted and cleaved with HindIII before cloning into the lambda vector Charon 21A. A sensitive miniblot hybridization method was used to monitor the physical and biochemical steps in the cloning procedure. Using this method, we have developed a highly efficient protocol for producing large numbers of recombinant phage from 0.2-1.0 X 10(6) sorted chromosomes. DNA from the following chromosomes was cloned: #4, 6, 8, 9, 11, 13, 14 + 15, 16, 17, 18, 19, 20, 21, 22 and Y. These libraries are available to the scientific research community and will be valuable in the genetic analysis of the human genome.
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