These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Improved synthesis and purification of methylated amanitins using diazomethane.
    Author: Little MC, Preston JF, Bonetti S.
    Journal: Int J Pept Protein Res; 1986 Sep; 28(3):282-8. PubMed ID: 3781742.
    Abstract:
    We have examined the conditions of methylating alpha-amanitin with diazomethane with the intent of producing 6'-O-methyl-alpha-amanitin (meAMA). Under the appropriate conditions meAMA was afforded as the sole product in nearly quantitative yield. By exceeding the stoichiometries designed for optimal meAMA synthesis, a dimethylated amanitin, 1'-N-, 6'-O-dimethyl-alpha-amanitin (dimeAMA), was also produced. Both products were characterized, following HPLC, by ultraviolet and n.m.r. spectroscopy. Based upon their inhibitory potential against wheat germ RNA polymerase II, apparent dissociation constants of 4.3 nM and 5.4 nM were estimated for meAMA and dimeAMA, respectively.
    [Abstract] [Full Text] [Related] [New Search]