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  • Title: Preparation of 3D nano silver trees/sea urchin-like gold and SERS detection of uric acid.
    Author: Lin X, Li LJ, Guo HY, Li R, Feng J.
    Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2024 Jan 15; 305():123464. PubMed ID: 37837927.
    Abstract:
    In this paper, 3D nano-silver trees/sea urchin-like gold Surface Enhanced Raman Spectroscopy (SERS) substrates were prepared by chemical reduction method, characterized by SEM, EDS, UV-Vis and XRD et.al, and the preparation process was optimized using Rhodamine B (RB) as the Raman signal molecule. The SERS characteristics of Uric Acid on this substrate were investigated and the results showed that the SERS substrate had a Raman enhancement factor of 1.9 × 107 for RB, and also had a significant Raman enhancement effect on Uric Acid. The SERS intensity (ISERS) at 1400 cm-1 showed a good linear relationship with the logarithm value of uric acid concentration (log C) between the range of 5 × 10-4 M to 1 × 10-7 M. The linear fitting equation was ISERS = 890 (log C) + 8066 (R2 = 0.983), and the LOD = 2.8 × 10-8 M. The spiked test was performed in human serum with recoveries ranging from 82.86% to 125.13% and RSD (n = 3) < 3.5%.
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