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  • Title: A method to evaluate the capacity of monocytes and macrophages to inhibit multiplication of an intracellular pathogen.
    Author: McLeod R, Remington JS.
    Journal: J Immunol Methods; 1979 May 10; 27(1):19-29. PubMed ID: 379229.
    Abstract:
    A new method to assess intracellular inhibition of multiplication or killing by normal and activated mouse peritoneal macrophages, human peripheral blood monocytes, and human monocyte-derived macrophages is described. This method involves measurement of incorporation of [3H]uracil into nucleic acids of the obligate intracellular parasite Toxoplasma gondii. The method utilizes the observation by Pfefferkorn and Pfefferkorn (1977) that [3H]uracil is incorporated in substantially greater amounts by T. gondii than by certain mammalian cell types. Differential uptake of [3H]uracil by Toxoplasma-infected and uninfected cultures allows for evaluation of the ability of macrophages or monocytes to inhibit or kill this organism. This method has been adapted to microsystem.
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