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  • Title: Phospholipid composition modifications influence phospholipase A2 activity in rat liver plasma membranes.
    Author: Momchilova AB, Petkova DH, Koumanov KS.
    Journal: Int J Biochem; 1986; 18(10):945-52. PubMed ID: 3792613.
    Abstract:
    The influence of the phospholipid composition and the physico-chemical properties of rat liver plasma membranes on the activity of membrane-bound phospholipase A2 has been investigated. The plasma membrane composition was modified by the aid of exogenous phospholipases A2, C and D, and by butanol treatment. The partially delipidated membranes thus obtained were enriched with different phospholipids. The steady-state fluorescent anisotropy of 1,6-diphenyl-1,3,5-hexatriene and the lipid order parameter-SDPH in the modified membranes were calculated. It was established that the activity of the membrane-bound phospholipase A2 was higher in rigid membranes and was decreased when the membrane lipid bilayer was fluidized.
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