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  • Title: Nontransferrin-bound serum iron in thalassemia and sickle cell patients.
    Author: Ahmed NK, Hanna M, Wang W.
    Journal: Int J Biochem; 1986; 18(10):953-6. PubMed ID: 3792614.
    Abstract:
    Nontransferrin-bound iron (NTBI) was separated from transferrin bound iron (TBI) by DEAE-Sephadex-CDS filtration. TBI is eluted with Tris-NaCl buffer, NTBI that is retained on the column is eluted with citric acid. NTBI was identified in serum from thalassemia and sickle cell patients. Normal serum contained less than 6% NTBI as compared with 15-18% in patient's sera. NTBI levels were decreased significantly after 8 hr chelation with deferoxamine (DFO).
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