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  • Title: A Novel Fluorescent and Colorimetric Method for the Determination of Formaldehyde Based on Albumin Nanoparticles-Polyethyleneimine-Ag+ Ion Nanohybrids.
    Author: Pang S, Zhong Q, Zhao Y, Xia N.
    Journal: J Fluoresc; 2024 Nov; 34(6):2871-2879. PubMed ID: 37938478.
    Abstract:
    As a carcinogenic substance, high dose of formaldehyde exposure may lead to poisoning and even death. Long-term exposure to low doses of formaldehyde can harm human skin, respiratory organs and immune system. Therefore, it is vital to detect formaldehyde content in real time. In this paper, a simple method for the determination of formaldehyde based on fluorometry and colorimetry was established in the range of 0-1.92 mg·mL-1. A fluorescence protein nanoparticles (BSA NPs) was prepared utlizing bovine serum albumin (BSA) as the raw material. Based on the silver mirror reaction, silver nanoparticles can be generated from the reaction between BSA NPs combined with polyethylenimide (PEI) and silver ion (Ag+) ions complex (BSA NPs-PEI-Ag) and formaldehyde. The fluorescent detection principle for formaldehyde was based on the fluorescence queching of BSA NPs-PEI-Ag system at 514 nm upon the reduction of Ag+ ions by formaldehyde. The colorimetric detection principle for formaldehyde was based on the enhancement of absorption band of BSA NPs-PEI-Ag system at 460 nm and color changes along with the generation of silver nanoparticles after the addition of formaldehyde. The proposed method was succesfully used for formaldehyde detection in real water sample with the recovery range of 106-110%.
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