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  • Title: [Radiation sensitivity of aerated and hypoxic clonogenic cells of Ehrlich ascites carcinoma].
    Author: Konopliannikov AG, Shteĭn LV, Kolesnikova AI.
    Journal: Med Radiol (Mosk); 1986 Dec; 31(12):68-70. PubMed ID: 3796262.
    Abstract:
    The authors presented comparative results on the survival of ascitic Ehrlich's carcinoma (AEC) clonogenic cells during exposure to 60Co gamma-beam radiation under the hypoxic and oxygenated conditions in vitro. A well-known method of hypoxia induction in dense cell suspensions by way of forced cell precipitation into a dense precipitate by a short-term suspension centrifugation was modified. Radiosensitivity of such hypoxic cells was characterized by a DO value of 6.59 +/- 0.29 Gy, and that of well oxygenated cells by 2.06 +/- 0.05 Gy. The oxygen enhancement ratio, determined as a ratio of DO values for hypoxic and oxygenated cells, was 3.2.
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