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  • Title: A study on the unprimed poly (dA-dT) synthesis catalyzed by preparations of E. coli DNA polymerase I.
    Author: Nazarenko IA, Bobko LE, Romashchenko AG, Khripin YL, Salganik RI.
    Journal: Nucleic Acids Res; 1979 Jun 11; 6(7):2545-60. PubMed ID: 379822.
    Abstract:
    Evidence was obtained indicating that the initiation of poly (dA-dT) de novo synthesis is provided by deoxynucleoside diphosphate: oligonucleotide deoxynucleotidyl transferase (dNDP-transferase present in preparations of E. coli DNA polymerase I and capable of catalyzing the unprimed polymerization of dNDP. dNDP-transferase synthesyzes short oligonucleotides which form template-primer complexes repeatedly replicated by DNA polymerase I. This conclusion was based on the following observations: the abolition of the lag period of poly (dA-dT) synthesis by preincubation of DNA-polymerase I preparations with dADP and dTDP; the presence of oligo (dA-dT) among the preincubation products; the suppressive effect of dithiothreitol and N-ethylmaleimide (inhibitors of dNDP-transferase) on the de novo, but not on the primed synthesis of poly (dA-dT), catalyzed by preparations of DNA-polymerase I.
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