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  • Title: Agroinoculation of Geminiviral Infectious Clones into Plants.
    Author: Morais IJ, Ferreira YFM, Nakasu EYT, Nagata T, Inoue-Nagata AK.
    Journal: Methods Mol Biol; 2024; 2724():65-70. PubMed ID: 37987898.
    Abstract:
    Most geminiviruses are not transmitted by mechanical inoculation. Therefore, pathogenicity and plant-pathogen interaction studies rely on agroinoculation using infectious clones, which involves cloning the geminiviral genome in a binary vector (see previous chapter for details). A suspension containing the infectious clone inserted into Agrobacterium tumefaciens cells is then inoculated into plants, i.e., agroinoculated. Below is a simple protocol for agroinoculation of an infectious geminivirus clone into plants.
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