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  • Title: Expression of the cloned S4 gene of reovirus serotype 3 in transformed eucaryotic cells: enrichment of the viral protein in the crude initiation factor fraction.
    Author: Lemay G, Millward S.
    Journal: Virus Res; 1986 Nov; 6(2):133-40. PubMed ID: 3799063.
    Abstract:
    The sigma 3 protein of reovirus is believed to play a role in the control of protein synthesis in reovirus-infected cells. In this paper we describe the establishment of a line of L-cells expressing the sigma 3 protein from the cloned S4 gene of reovirus serotype 3, under the control of the SV40 early promoter. The protein was enriched in the crude initiation factor fraction prepared by a high-salt wash of ribosomes. There was no apparent detrimental effect on the cell line.
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