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  • Title: An enzymatic method for the synthesis of [14C]pyridoxal 5'-phosphate from [14C]pyridoxine.
    Author: Yagi T, Takasugi M, Yamamoto S, Nozaki M.
    Journal: Anal Biochem; 1986 Oct; 158(1):36-41. PubMed ID: 3799967.
    Abstract:
    A new enzymatic method for the synthesis of [14C]pyridoxal 5'-phosphate is presented. [14C]Pyridoxal 5'-phosphate was synthesized from [14C]pyridoxine through the successive actions of pyridoxal kinase and pyridoxamine 5'-phosphate oxidase in a reaction mixture containing ATP, [14C]pyridoxine, and both enzymes. [14C]Pyridoxal 5'-phosphate was isolated by omega-aminohexyl-Sepharose 6B column chromatography. The overall yield of the product was more than 60%, starting from 550 nmol of [14C]pyridoxine. The radiochemical purity of the products, as determined by thin-layer and ion-exchange chromatography, was greater than 98%.
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