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  • Title: Gnotobiotic calves: derivation, maintenance, Thiry-Vella loop preparation, and ethylene oxide gas sterilization.
    Author: Newman LE, Whitehair CK, Mullaney TP.
    Journal: Am J Vet Res; 1986 Dec; 47(12):2632-6. PubMed ID: 3800124.
    Abstract:
    Twenty-four pregnant Holstein cows were used to develop techniques and procedures to obtain calves by hysterotomy and to maintain them in a closed gnotobiotic environment (sterile plastic-film isolators) for as long as 10 days for infectious disease research. Thiry-Vella loop preparation for obtaining intestinal loop secretions was done in a surgical isolator and was successful in 12 of 14 gnotobiotic calves. Ethylene oxide gas was effective in sterilizing heat-labile and moisture-sensitive instruments and equipment.
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