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  • Title: Construction of a highly sensitive detection platform for heparin based on a "turn-off" cationic fluorescent dye.
    Author: Liu Y, Zhang Y, Liu C, Wang C, Xu B, Zhao L.
    Journal: Spectrochim Acta A Mol Biomol Spectrosc; 2024 Mar 05; 308():123670. PubMed ID: 38006866.
    Abstract:
    A highly sensitive detection platform for heparin was constructed via the utilization of a commercially available cationic fluorescent dye (cresyl violet acetate, CV) as a fluorescence probe. The electrostatic binding between CV and heparin quenched the fluorescence in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic (HEPES) buffer solution (10 mM, pH 7.1). CV was highly selective towards heparin over other potential inferring substances. The detection limit of heparin detection was 5.19 ng/mL, and the linear working range was 0 ∼ 1 μg/mL in HEPES solution. In 1 % serum, the detection platform based on the fluorescence "turn-off" behavior of CV was also successfully constructed with a detection limit of 5.86 ng/mL in the linear range of 0 ∼ 0.8 μg/mL. Moreover, the CV-heparin complex was considered a potential sensor platform for the detection of protamine because of its stronger affinity for heparin and protamine.
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