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  • Title: Cytosol glucocorticoid receptor in the neoplastic epithelial duct cell line from human salivary gland (HSG).
    Author: Kurokawa R, Hatakeyama S, Kyakumoto S, Ota M.
    Journal: Biochem Int; 1986 Oct; 13(4):671-9. PubMed ID: 3801040.
    Abstract:
    Neoplastic epithelial duct cell line from human salivary gland (HSG cell) contained cytosol glucocorticoid receptor. Scatchard analysis of cytosol indicated that the dissociation constant (Kd) was 5.6-6.5 nmol/l and the number of binding sites was 83-92 fmol/mg protein. A competitive assay showed that the binding sites for [3H]triamcinolone acetonide were specific to glucocorticoid. Glycerol density gradient centrifugation displayed that the [3H]triamcinolone acetonide receptor complexes sedimented in the 8.5 S region under low salt conditions and in the 4.2 S region under high salt condition (0.4 M KCl). The same high salt conditions induced an increased binding of [3H]triamcinolone acetonide complexes for DNA-cellulose.
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