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  • Title: Urotensin II lowers cytoplasmic free calcium concentration in goby enterocytes: measurements using quin2.
    Author: Loretz CA, Assad JA.
    Journal: Gen Comp Endocrinol; 1986 Dec; 64(3):355-61. PubMed ID: 3803890.
    Abstract:
    A technique is described for the measurement of cytoplasmic free calcium concentration ([Ca2+]c) in a suspension of goby intestinal epithelial cells using the fluorescent probe quin2. For 18 cell suspensions, [Ca2+]c was determined to be 142 +/- 14 nM. The caudal neurosecretory peptide urotensin II at 10(-7) to 10(-6) M concentration reduced [Ca2+]c by 20-50%. Together with previous findings linking the calcium-calmodulin system with the stimulation of Na+ and Cl- absorption in this tissue, it appears that alterations in cytoplasmic Ca2+ may mediate urotensin II-induced stimulation of ion transport.
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