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  • Title: Titrimetric assay of pancreatic lipase in pharmaceutical preparations.
    Author: Lecat D, Letavernier JF, Bourquin T, Soroste M, Vaucher D.
    Journal: J Assoc Off Anal Chem; 1986; 69(6):1022-5. PubMed ID: 3804942.
    Abstract:
    Lyophilized pancreas and pancreatic extracts are widely used in therapy of pancreatic exocrine function deficiencies. Among the measures for quality of the extracts, assay for lipolytic activity appears to be one of the best. However, assay precision is poor, because the specificity and mode of action of lipase requires careful optimization of the assay parameters, especially substrate and measurement conditions. In the method proposed here, substrate quality is improved by the use of sodium desoxycholate and a high-speed stirrer for more reproducible emulsions. Fatty acids are assayed by a titrimetric method, using an electronically monitored pH meter. A comparative statistical study of the U.S. Pharmacopeia method, the International Pharmaceutical Federation (FIP) method, and a modified FIP method showed the latter to have improved accuracy and reproducibility.
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