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  • Title: Macrophage cell line, J774, producing a tumor necrosis factor.
    Author: Sakurai A, Satomi N, Haranaka K.
    Journal: Jpn J Exp Med; 1986 Oct; 56(5):195-9. PubMed ID: 3807015.
    Abstract:
    Cytotoxic factor was produced from murine macrophage-like cell line, J774, after stimulation with 12-o-tetradecanoyl phorbol-13-acetate (TPA) and lipopolysaccharide (LPS). J774-derived cytotoxic factor is a glycoprotein with a molecular weight of 39,000 by gel filtration and 18,000 by SDS-PAGE, and with an isoelectric point of below 4.3. J774-derived cytotoxic factor exhibited cytotoxicity to L(S) cells, not cytotoxic to L(R) cells, and a necrotizing action against transplanted Meth A sarcoma. J774-derived cytotoxic factor and murine serum tumor necrosis factor (TNF) were identical as regards properties.
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