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  • Title: [Effect of various methods of the preservation and preparation for study of human peripheral blood on erythrocyte morphology (scanning electron microscopy)].
    Author: Mechenov G, Dimitrov M.
    Journal: Eksp Med Morfol; 1979; 18(2):106-12. PubMed ID: 380965.
    Abstract:
    In the paper the authors describe the influence of various ways of preservation and preparation of peripheral human blood on the morphology of erythrocytes. The investigation was performed by a scanning electrone microscope JSM-35 JEOL--Japan). The basic types of erythrocytes were shown, which were encountered in the samples. It was established that dehydratation and various methods for fixation of erythrocytes did not affect their morphology. The authors found changes, which occurred in erythrocyte morphology after blood preservation in anticoagulant,FBFR and as a whole blood at various intervals. The action of centrifugal forces in the process of centrifugation of blood on various types of erythocytes was indicated as well. The results of the studies were illustrated with 21 pictures, made at magnification of 3000 X up to 15 000 X.
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