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  • Title: [A rapid method for the determination of cyclosporin A in blood by high performance liquid chromatography].
    Author: Toulet V, Saux MC, Rouquette C, Penouil F, Brachet-Liermain A.
    Journal: Ann Biol Clin (Paris); 1986; 44(5):517-21. PubMed ID: 3813137.
    Abstract:
    The purpose of this paper is to propose a method for the determination of cyclosporin in whole blood by means of HPLC with UV detection. After a one step extraction into ether, the organic layer is washed NaOH and evaporated to dryness. The residue is reconstituted in a mixture of acetonitrile and water (40/60) (V/V) and injected into the chromatograph. The C8 reversed phase column is warming at 75 degrees C temperature. The mobile phase consists of acetonitrile and water (60/40) (V/V) and the wavelength is set at 210 nm. The technique is rapid, sensitive, reproducible and well suited for therapeutic monitoring and for pharmacokinetic studies.
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