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  • Title: Metabolism of 17-hydroxyprogesterone by a Bacillus species.
    Author: Mahato SB, Banerjee S.
    Journal: Biochem J; 1986 Oct 15; 239(2):473-6. PubMed ID: 3814085.
    Abstract:
    Fermentation of 17-hydroxyprogesterone with a Bacillus species (IICB-301) in a modified nutrient medium under aerobic conditions yielded androst-4-ene-3,17-dione and 15 alpha,17-dihydroxypregn-4-ene-3,20-dione in addition to a new pregnane analogue, 6 beta,17,20 alpha-trihydroxypregn-4-ene-3-one. Each microbial metabolite was characterized by the application of various spectroscopic techniques. The availability of the new metabolite, 6 beta,17,20 alpha-trihydroxypregn-4-ene-3-one, enabled complete elucidation of its 13C-n.m.r. spectrum.
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