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  • Title: Relative spectral response as a function of sequential ligand binding.
    Author: Deerfield DW, Hoke RA, Pedersen LG, Darden T, Hiskey RG.
    Journal: Biochem Biophys Res Commun; 1986 Dec 30; 141(3):1207-12. PubMed ID: 3814120.
    Abstract:
    A unique method is presented for the determination of the critical number of ligands that must bind to a macromolecule to elicit a spectroscopic response. This method is based on analysis of ligand binding data. For example, four Ca2+ and two Mg2+ ions are necessary for mirroring the relative decrease in the intrinsic fluorescence of bovine prothrombin fragment 1. For application of the method, ligand loading and relative spectroscopic response data must be measured over a full range of concentrations.
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