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  • Title: Facile release of NADPH oxidase from polymorphonuclear leukocyte membrane by mild pressure treatment.
    Author: Katayama T, Okamura N, Ishibashi S.
    Journal: J Biochem; 1986 Oct; 100(4):1087-9. PubMed ID: 3818561.
    Abstract:
    NADPH oxidase, a complex enzyme system in the cell membrane responsible for the bactericidal function of polymorphonuclear leukocytes through the production of superoxide anion, was facilely released by mild treatment with a press. At the pressure where almost all NADPH oxidase activity was released, releases of the activities of lactate dehydrogenase, 5'-nucleotidase, lysozyme, and N-acetyl-beta-glucosaminidase, and of the amount of total protein were negligible. This method can be useful for the elucidation of NADPH oxidase.
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