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  • Title: A simple technique for increased sensitivity in immunoelectrophoresis by the use of antiserum against IgG.
    Author: Samuelson L, Ohman R, Ridell M.
    Journal: J Immunol Methods; 1987 Mar 12; 97(2):281-3. PubMed ID: 3819446.
    Abstract:
    Precipitates in two-dimensional immunoelectrophoresis (2D-IE) plates can be amplified by the application of an antiserum against IgG after the second dimension run. Thereby, the sensitivity of the IE method is increased; faint precipitates and antigen-antibody reactions close to but below the level of forming recognizable precipitates are made visible. The advantage of the method was demonstrated for a multilinear Mycobacterium phlei system.
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