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  • Title: General characteristics, molecular and genetic analysis of two new UV-sensitive mutants of Chlamydomonas reinhardtii.
    Author: Vlcek D, Podstavkova S, Miadokova E, Adams GM, Small GD.
    Journal: Mutat Res; 1987 Mar; 183(2):169-75. PubMed ID: 3821764.
    Abstract:
    Two new UV-sensitive mutants of Chlamydomonas, UVS10 and UVS11, were isolated. Both behave as single nuclear mutations. UVS10 was mapped to linkage group I. UVS11 is a separate, unlinked mutation but has not yet been located to a specific linkage group. Both mutants are proficient in the excision of pyrimidine dimers from nuclear DNA. The survival of UV-irradiated UVS11 is increased when plated in the presence of 1.5 mM caffeine, similar to wild-type. Caffeine has no effect on the survival of UV-irradiated UVS10. UV-irradiated UVS11 frequently divides at least once before dying, in contrast to UVS10 or wild-type. UVS11 also exhibits a much increased frequency of mutation to streptomycin resistance after UV irradiation.
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