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  • Title: Antimalarial target vulnerability of the putative Plasmodium falciparum methionine synthase.
    Author: Leela N, Prommana P, Kamchonwongpaisan S, Taechalertpaisarn T, Shaw PJ.
    Journal: PeerJ; 2024; 12():e16595. PubMed ID: 38239295.
    Abstract:
    BACKGROUND: Plasmodium falciparum possesses a cobalamin-dependent methionine synthase (MS). MS is putatively encoded by the PF3D7_1233700 gene, which is orthologous and syntenic in Plasmodium. However, its vulnerability as an antimalarial target has not been assessed. METHODS: We edited the PF3D7_1233700 and PF3D7_0417200 (dihydrofolate reductase-thymidylate synthase, DHFR-TS) genes and obtained transgenic P. falciparum parasites expressing epitope-tagged target proteins under the control of the glmS ribozyme. Conditional loss-of-function mutants were obtained by treating transgenic parasites with glucosamine. RESULTS: DHFR-TS, but not MS mutants showed a significant proliferation defect over 96 h, suggesting that P. falciparum MS is not a vulnerable antimalarial target.
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