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  • Title: Native opioid-like peptides in Squilla mantis ganglia.
    Author: Marino G, Palmisano A, Di Marzo V, Melck D, Miralto A.
    Journal: Peptides; 1985; 6 Suppl 3():403-6. PubMed ID: 3831968.
    Abstract:
    To detect the presence of a mammalian-like enkephalin precursor in suboesophageal ganglia of Squilla mantis, an arthropod shown to be sensitive in vivo to opiates [8], protein acid extracts were fractionated by gel filtration into three large pools: A(Mr greater than 65,000), B(10,000 less than Mr less than 65,000) and C(Mr less than 10,000). Only the low molecular weight pool, pool C, showed opioid-like activity when assayed by displacing labeled D-Ala2-D-Leu5-enkephalin from rat brain membranes. After trypsin and carboxypeptidase B proteolysis, pool A remained inactive, while pool B turned out to be active and was shown to inhibit the twitch response of electrically stimulated guinea-pig ileum. After HPLC fractionation of proteolyzed pool B, most of the opioid-like activity was found to be associated with a fraction showing an elution volume different from that of opioid peptide standards. Furthermore, no fraction showed immunoreactivity with anti-Met-enkephalin antibodies. The results suggest that native opioid-like peptides are present in Squilla mantis and are most likely released from higher molecular weight precursor(s).
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