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  • Title: Effect of cytosol on DNA synthesis in isolated mammary gland nuclei from rabbits during pregnancy and early lactation.
    Author: Kleczkowska D, Zwierzchowski L.
    Journal: Acta Biochim Pol; 1985; 32(4):305-17. PubMed ID: 3832703.
    Abstract:
    The in vitro DNA synthesis of nuclei isolated from rabbit mammary gland was stimulated by a cytosol fraction from the same tissue. Time-course of this phenomenon was followed during rabbit pregnancy at 5-day intervals. The stimulation was shown to be physiological state-dependent in that it could be detected only in the case of cytosols prepared on day-10 of pregnancy and from day-20 or 25 of pregnancy through day-5 of lactation. Moreover, only nuclei isolated on days-15 or -30 of pregnancy responded to the exogenously added cytosol. The DNA-stimulating activity was partially characterized. It was shown to be protein-like since it was heat-labile, mostly non-dialysable and sensitive to N-ethylmaleimide and trypsin treatment. Sedimentation analysis on sucrose density gradients separated this activity into 3-4 peaks, distinct from the cytoplasmic DNA polymerase.
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