These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Quantitative changes in histones during the formation of structures characteristic of the male pronucleus in mammalian spermatozoa in vitro].
    Author: Zakhidov ST, Boronchuk GV, Nauk VA, Brodskiĭ VIa.
    Journal: Ontogenez; 1985; 16(1):73-5. PubMed ID: 3838375.
    Abstract:
    Treatment of bull spermatozoa with DDC--Na/dithiothreitol results in the swelling and decondensation of nuclear chromatin. The structures formed at the final stages of decondensation are morphologically similar to the male pronucleus. Cytophotometric analysis has shown that decondensation of chromatin in the gametes in followed by quantitative changes of basic nuclear proteins. In partly--decondensed sperm nuclei the intensity of histone staining increases as a result of the appearance of extra reactive groups. In fully decondensed nuclei there remain only 54% of histones of the original haploid level. Nucleoproteins revealed in the sperm with fully dispersed chromatin must be histones of the somatic type.
    [Abstract] [Full Text] [Related] [New Search]