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  • Title: An enzyme immunoassay (EIA) for progesterone in horse plasma.
    Author: Seeger K, Thurow H, Haede W, Knapp E.
    Journal: J Immunol Methods; 1979; 28(3-4):211-7. PubMed ID: 383843.
    Abstract:
    A simple enzyme immunoassay (EIA) for the measurement of progesterone is described. Antibody against 11-OH-hemisuccinate-BSA is bound to polystyrene tubes. 11-OH-hemisuccinyl-beta-D-galactosidase is used as enzyme-coupled antigen and methylumbelliferyl-beta-D-galactoside as substrate. Concentrations down to 0.156 ng/ml plasm or amounts of 93 pg/tube are detectable. Probit analysis gave a linear relationship between log concentration and percentage of binding. A comparison of EIA and radioimmunoassay gave a correlation coefficient of 0.81. The assay is sufficiently sensitive to estimate progesterone levels in plasma.
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