These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Laboratory and clinical evaluation of a rapid slide agglutination test for phosphatidylglycerol.
    Author: Halvorsen PR, Gross TL.
    Journal: Am J Obstet Gynecol; 1985 Apr 15; 151(8):1061-6. PubMed ID: 3838619.
    Abstract:
    Amniostat-FLM is a recently developed rapid slide agglutination test for amniotic fluid phosphatidylglycerol. We compared phosphatidylglycerol quantitated by two-dimensional thin-layer chromatography with phosphatidylglycerol determined by Amniostat-FLM in 180 amniotic fluid samples. If any level of phosphatidylglycerol by thin-layer chromatography, including a trace, was considered positive, then phosphatidylglycerol by thin-layer chromatography and Amniostat-FLM were concordant in 171 of 180 samples (95%). Both were positive in 123 and both negative in 48 samples. The nine amniotic fluid samples with discrepant results were as follows: Five negative Amniostat-FLM results were associated with trace phosphatidylglycerol by thin-layer chromatography (1% to 2% of total phospholipids), one negative Amniostat-FLM result was associated with 3% phosphatidylglycerol by thin-layer chromatography, and three positive Amniostat-FLM results were associated with negative phosphatidylglycerol by thin-layer chromatography results. There were 14 samples with trace phosphatidylglycerol by thin-layer chromatography; of these, there were nine positive and five negative Amniostat-FLM results. If greater than 2% phosphatidylglycerol by thin-layer chromatography was considered positive, concordance between the two tests was 93% (167 of 180). From these samples, 119 infants were delivered within 72 hours of amniocentesis. There were 100 positive Amniostat-FLM results and all were associated with the absence of respiratory distress syndrome; of the 19 negative Amniostat-FLM results, five were associated with infants who developed respiratory distress syndrome. The present study confirms that Amniostat-FLM is a simple, rapid, and accurate test for determining the presence of phosphatidylglycerol in amniotic fluid.
    [Abstract] [Full Text] [Related] [New Search]