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  • Title: Release of NPY-like immunoreactive material from primary cultures of chromaffin cells prepared from bovine adrenal medulla.
    Author: Kataoka Y, Majane EA, Yang HY.
    Journal: Neuropharmacology; 1985 Jul; 24(7):693-5. PubMed ID: 3839572.
    Abstract:
    In primary cultures of bovine chromaffin cells, the cell contents of NPY immunoreactive material was found to be stable for about 4 days. When these cells were transferred to the Locke's solution from the culture medium, NPY immunoreactive material was released spontaneously. Addition of potassium (45 mM) resulted in a 3-fold increase in the rate of NPY release in a calcium dependent fashion. NPY immunoreactivity in chromaffin cells is composed of NPY, a lesser immunoreactive peptide and two very minor immunoreactivities. The same immunoreactive peptides were released by 45 mM potassium.
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