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  • Title: Accumulation of creatine kinase mRNA during myogenesis: molecular cloning of a B-creatine kinase cDNA.
    Author: Kwiatkowski RW, Ehrismann R, Schweinfest CW, Dottin RP.
    Journal: Dev Biol; 1985 Nov; 112(1):84-8. PubMed ID: 3840441.
    Abstract:
    Cytosolic creatine kinase isoenzymes MM, MB, and BB are assembled from M or B subunits which occur in different relative amounts in specific tissues. The accumulation of mRNAs encoding the M and B subunits was measured during myogenesis in culture. The relative concentration of the two mRNAs was determined by hybridization with a M-CK cDNA probe isolated previously and a B-CK cDNA probe, the cloning and characterization of which is reported here. The B-CK cDNA hybridizes specifically to a 1.6-kb mRNA found in brain and gizzard but not in adult skeletal muscle tissue. The M-CK cDNA hybridizes to a smaller mRNA 1.4-kb long which is specific to skeletal muscle. In culture, the B-CK mRNA is transiently induced and then declines to a low but detectable level.
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