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  • Title: Development and Validation of UPLC Tandem Mass Spectrometry Assay for Ceftibuten and Sulbactam in Human Plasma.
    Author: Valero-Rivera KP, Magaña-Aquino M, Del Carmen Romero-Méndez M, Morales-Barragán MN, Sagahón-Azúa J, Medellín-Garibay SE, Del Carmen Milán-Segovia R, Martínez-Gutiérrez F, Romano-Moreno S.
    Journal: J Chromatogr Sci; 2024 Oct 01; 62(8):783-788. PubMed ID: 38521966.
    Abstract:
    A sensitive and rapid ultra-performance liquid chromatography coupled with -tandem mass spectrometry (UPLC-MS/MS) method was developed and validated to determine ceftibuten (CTB) and sulbactam (SUL) in human plasma. An ACQUITY UPLC HSS T3 C18 (2.1 × 100 mm), 1.8 μm column with gradient elution of water (0.1% formic acid) and acetonitrile was used for separation at a flow rate of 0.2 mL/min. This method involves a simple sample preparation with acetonitrile. The calibration curves of CTB and SUL in plasma showed good linearity over the concentration range of 0.50-25 μg/mL and with a correlation coefficient (r2) >0.99. This method was validated in terms of selectivity, linearity, precision, accuracy and stability. High precision was obtained with coefficients of variation <15%. Excellent recovery in the range of 90-104% was achieved for CTB and SUL was 86-110%. The method has the potential utility to support pharmacometric modeling in clinical practice and biopharmaceutic studies.
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