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  • Title: A single step procedure for purification of estradiol 17 beta-dehydrogenase from human placenta.
    Author: Mendoza-Hernández G, Rendón JL, Díaz-Zagoya JC.
    Journal: Biochem Biophys Res Commun; 1985 Jan 16; 126(1):477-81. PubMed ID: 3855619.
    Abstract:
    A new and simple procedure for purification to homogeneity of the soluble estradiol 17 beta-dehydrogenase [E C.1.1.1.62] from human placenta has been developed. The purification is achieved in a single step by affinity-absorption of Cibachrome Blue F3G-A coupled to Sepharose and selective elution with NADP+. Homogeneous estradiol 17 beta-dehydrogenase has a specific activity of 7.2 units/mg. and has been purified 2400-fold with a 93% recovery.
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