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  • Title: The cytosolic phorboid receptor correlates with hormone dependency in six mammary carcinoma cell lines.
    Author: Costa SD, Fabbro D, Regazzi R, Küng W, Eppenberger U.
    Journal: Biochem Biophys Res Commun; 1985 Dec 17; 133(2):814-22. PubMed ID: 3866578.
    Abstract:
    Potent, structurally different tumor promoters inhibited growth of 6 human mammary carcinoma cell lines (ROOS et al, PNAS in press). This growth inhibition was investigated by measuring the phorboid receptor binding using [3H] PDBu (4 beta-phorbol 12, 13 dibutyrate). Specific, high affinity receptors were found in all six cell lines. [3H] PDBu binding affinities were higher in the cytosolic fractions than in the corresponding intact cells (K alpha = app. 1nM vs K alpha = app. 15nM). The hormone-independent cell lines (BT-20, HBL-100 and MDA-MB-231) exhibited significantly higher levels of cytosolic [3H] PDBu receptors than the hormone-dependent cells (MCF-7, T-47-D and ZR-75-1). The subcellular distribution of the [3H] PDBu binding correlated well with the distribution of the protein kinase C activity (r = 0.95).
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