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  • Title: Research Note: Rapid generation of a novel fowl adenovirus serotype 11 reverse genetic platform.
    Author: Qiao Q, Yang P, Liu J, Li Y, Li X, Qiu L, Xiang M, Zhu Y, Cong Y, Wang Z, Li J, Wang B, Zhao J.
    Journal: Poult Sci; 2024 Jun; 103(6):103745. PubMed ID: 38670058.
    Abstract:
    Fowl adenovirus serotype 11 (FAdV-11) is one of the main causative agents of inclusion body hepatitis (IBH) in broilers. Outbreaks of FAdV-11-related IBH have been increasingly reported in China and many other geographical areas worldwide. However, the critical virulence factors of FAdV-11 remain uncertain due to the lack of technical platforms for efficient manipulation of FAdV-11 genome. Here, we reported the establishment of a FAdV-11 reverse genetic system based on a novel FAdV-11 Chinese isolate FJSW/2021 using the exonuclease combined with RecET (ExoCET), Redαβ recombineering and ccdB counter-selection techniques for the first time. A recombinant FAdV-11 was rescued efficiently by using the established reverse genetic platform through swapping the ORF11 gene of the FAdV-11 FJSW/2021 with the ZsGreen fluorescent protein expression cassette. This study provides an effective technical platform for identifying virulence factors of FAdV-11 and developing recombinant FAdV-11-vectored vaccine candidates.
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