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  • Title: A direct spectrophotometric assay for D-alanine carboxypeptidases and for the esterase activity of beta-lactamases.
    Author: Pratt RF, Faraci WS, Govardhan CP.
    Journal: Anal Biochem; 1985 Jan; 144(1):204-6. PubMed ID: 3872609.
    Abstract:
    A direct spectrophotometric pH indicator method has been devised to assay the activity of the D-Ala carboxypeptidase/transpeptidase of Streptomyces R61, and which should be of general application to D-Ala carboxypeptidases. The substrate employed is N,N'-diacetyl-L-lysyl-D-alanyl-D-lactate. The method allows the determination of steady-state kinetic parameters, and can also be used for the assay of the esterase activity of beta-lactamases against specific depsipeptides.
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