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  • Title: "Thiocyanate gold": small (2-3 nm) colloidal gold for affinity cytochemical labeling in electron microscopy.
    Author: Baschong W, Lucocq JM, Roth J.
    Journal: Histochemistry; 1985; 83(5):409-11. PubMed ID: 3878348.
    Abstract:
    Reduction of HAuCl4 by NaSCN or KSCN produces colloidal gold particles of 2.6 nm in diameter and homogeneous in size (coefficient of variation approximately 15%). The AuSCN sol forms protein-gold complexes. The amount of protein required to form an AuSCN-protein complex is best determined in the electron microscope, where serial dilutions of protein with gold sol are inspected for the presence of aggregates. By immuno-electron microscopy SCN-gold complexed to protein A is active and visible as is shown by revealing alpha-amylase in rat pancreatic acinar cells.
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