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  • Title: Isolation of haemin-requiring mutants of Escherichia coli K12.
    Author: McConville ML, Charles HP.
    Journal: J Gen Microbiol; 1979 Jul; 113(1):155-64. PubMed ID: 387909.
    Abstract:
    Fifty-five haemin-requiring mutants were isolated from haemin-permeable mutants. According to their growth responses to haem precursors and their patterns of porphyrin accumulation, the 55 mutants fell into three groups which were judged to have defects in 5-aminolaevulinate dehydratase, ferrochelatase, and uroporphyrinogen III cosynthase or uroporphyrinogen decarboxylase. In mutants of the group deficient in 5-aminolaevulinate dehydratase, the mutations were adjacent to lac, and evidence is presented that the mutations were in hemB and were commonly deletions extending into proC.
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